miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32312 5' -54 NC_007024.1 + 23058 0.66 0.766627
Target:  5'- uUCUUGAUGCCgGcAUUGGCacccaucGgaACGCg -3'
miRNA:   3'- -AGAGCUACGGgUcUAACCG-------CgaUGCG- -5'
32312 5' -54 NC_007024.1 + 54877 0.66 0.757334
Target:  5'- --gUGGUGCCCAGGgcaucCGCUGCGg -3'
miRNA:   3'- agaGCUACGGGUCUaacc-GCGAUGCg -5'
32312 5' -54 NC_007024.1 + 14287 0.67 0.693094
Target:  5'- cCUU--UGCCCAGcccUUGG-GCUGCGCc -3'
miRNA:   3'- aGAGcuACGGGUCu--AACCgCGAUGCG- -5'
32312 5' -54 NC_007024.1 + 39779 0.68 0.648907
Target:  5'- gCUCGAUGCCauuauGAcccuaaagcUGGCGCUuaaauugcaaGCGCa -3'
miRNA:   3'- aGAGCUACGGgu---CUa--------ACCGCGA----------UGCG- -5'
32312 5' -54 NC_007024.1 + 22219 0.69 0.626675
Target:  5'- gUCUUGAUGCUCGGcaUGGCGCccaaGUc -3'
miRNA:   3'- -AGAGCUACGGGUCuaACCGCGaug-CG- -5'
32312 5' -54 NC_007024.1 + 24665 0.72 0.406848
Target:  5'- aUCUgGAUGCCCAGcucauuUGCUGCGCu -3'
miRNA:   3'- -AGAgCUACGGGUCuaacc-GCGAUGCG- -5'
32312 5' -54 NC_007024.1 + 21967 1.12 0.000763
Target:  5'- aUCUCGAUGCCCAGAUUGGCGCUACGCu -3'
miRNA:   3'- -AGAGCUACGGGUCUAACCGCGAUGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.