Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32314 | 5' | -52.4 | NC_007024.1 | + | 10477 | 0.66 | 0.86716 |
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Target: 5'- cGGUGGUAUACGUGAU--UAacuGCCc -3' miRNA: 3'- -CCGCCGUAUGCACUGgaAUguuCGGc -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 28663 | 0.67 | 0.823419 |
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Target: 5'- uGCGaGUAaggGCGUGACCUUggACGAuGCCc -3' miRNA: 3'- cCGC-CGUa--UGCACUGGAA--UGUU-CGGc -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 17208 | 0.67 | 0.814009 |
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Target: 5'- uGCGGUGUAgG-GACUUgaUGCGAGCUGc -3' miRNA: 3'- cCGCCGUAUgCaCUGGA--AUGUUCGGC- -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 9851 | 0.7 | 0.666808 |
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Target: 5'- cGGCGGCcgGCuUGGCCggaGCA-GCCu -3' miRNA: 3'- -CCGCCGuaUGcACUGGaa-UGUuCGGc -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 12653 | 0.72 | 0.533785 |
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Target: 5'- uGGCcuGGC--ACGUGAUCUUGCGcaggacAGCCGg -3' miRNA: 3'- -CCG--CCGuaUGCACUGGAAUGU------UCGGC- -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 55110 | 0.72 | 0.51232 |
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Target: 5'- cGGCGGUcuugGCGUucuGGCC--GCGAGCCGa -3' miRNA: 3'- -CCGCCGua--UGCA---CUGGaaUGUUCGGC- -5' |
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| 32314 | 5' | -52.4 | NC_007024.1 | + | 22785 | 1.12 | 0.001249 |
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Target: 5'- uGGCGGCAUACGUGACCUUACAAGCCGg -3' miRNA: 3'- -CCGCCGUAUGCACUGGAAUGUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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