Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32321 | 5' | -55.3 | NC_007024.1 | + | 51991 | 0.66 | 0.711421 |
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Target: 5'- uGGagUCGGCCagGCA-CgACCAcaugGCGGCAAc -3' miRNA: 3'- -CCa-AGCCGG--UGUaGgUGGU----UGCCGUU- -5' |
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| 32321 | 5' | -55.3 | NC_007024.1 | + | 8621 | 0.67 | 0.612309 |
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Target: 5'- uGGUgaaggUGGCCggauucuugGCGgugaugguaCCACCGACGGCGAg -3' miRNA: 3'- -CCAa----GCCGG---------UGUa--------GGUGGUUGCCGUU- -5' |
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| 32321 | 5' | -55.3 | NC_007024.1 | + | 40219 | 0.68 | 0.590214 |
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Target: 5'- ---cUGGCgGC-UCCGCCAAgGGCGAu -3' miRNA: 3'- ccaaGCCGgUGuAGGUGGUUgCCGUU- -5' |
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| 32321 | 5' | -55.3 | NC_007024.1 | + | 9701 | 0.69 | 0.535749 |
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Target: 5'- cGGUgcCGGUCACAgaggggucgCCggcagcuuuGCCAGCGGCAAc -3' miRNA: 3'- -CCAa-GCCGGUGUa--------GG---------UGGUUGCCGUU- -5' |
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| 32321 | 5' | -55.3 | NC_007024.1 | + | 46444 | 0.69 | 0.493478 |
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Target: 5'- aGGUUCGG--GCGUCgGCCAACGGa-- -3' miRNA: 3'- -CCAAGCCggUGUAGgUGGUUGCCguu -5' |
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| 32321 | 5' | -55.3 | NC_007024.1 | + | 33985 | 1.09 | 0.000953 |
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Target: 5'- cGGUUCGGCCACAUCCACCAACGGCAAg -3' miRNA: 3'- -CCAAGCCGGUGUAGGUGGUUGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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