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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32381 | 5' | -53.4 | NC_007041.1 | + | 5015 | 0.74 | 0.085343 |
|
Target: 5'- -aUGAuuacCCAGGCUUGGGCCAAGaCUGa -3' miRNA: 3'- caACUu---GGUUCGGACUCGGUUCgGAC- -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4937 | 0.7 | 0.184347 |
|
Target: 5'- --aGAuaACCuAGCCUuGGCCAAGCCg- -3' miRNA: 3'- caaCU--UGGuUCGGAcUCGGUUCGGac -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4465 | 0.79 | 0.040835 |
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Target: 5'- -aUGGGCCAAGUCUGgcaaccaagcaugGGCCAGGUCUGg -3' miRNA: 3'- caACUUGGUUCGGAC-------------UCGGUUCGGAC- -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4371 | 0.73 | 0.114494 |
|
Target: 5'- -cUGGguGCCAAGCCUGGuuGCCAGcCCUGa -3' miRNA: 3'- caACU--UGGUUCGGACU--CGGUUcGGAC- -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4314 | 0.96 | 0.001645 |
|
Target: 5'- aGUUGAACCAAGCCUGA-CCAAGCCUGg -3' miRNA: 3'- -CAACUUGGUUCGGACUcGGUUCGGAC- -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4313 | 0.75 | 0.079899 |
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Target: 5'- -cUGGACCAAGCUUGuaAGCCA-GCCUu -3' miRNA: 3'- caACUUGGUUCGGAC--UCGGUuCGGAc -5' |
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| 32381 | 5' | -53.4 | NC_007041.1 | + | 4270 | 0.66 | 0.324204 |
|
Target: 5'- --cGGGCCcAGGCUUG-GCuCAGGCUUGg -3' miRNA: 3'- caaCUUGG-UUCGGACuCG-GUUCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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