Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32385 | 5' | -52.1 | NC_007041.1 | + | 10524 | 0.67 | 0.387636 |
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Target: 5'- cUCGGUCUUGGCUU-GG-CCAGagGUc -3' miRNA: 3'- -AGUCGGAAUCGAAuCCaGGUCagCAc -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5334 | 0.85 | 0.017381 |
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Target: 5'- gUCGGCCcUAGCUaAGGcCCAGUCGUGg -3' miRNA: 3'- -AGUCGGaAUCGAaUCCaGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5542 | 1.01 | 0.001085 |
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Target: 5'- gUCGGCCcUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGaAUCGAAUCCAGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5438 | 1.01 | 0.001085 |
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Target: 5'- gUCGGCCcUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGaAUCGAAUCCAGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5646 | 1.1 | 0.000209 |
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Target: 5'- gUCAGCCUUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGAAUCGAAUCCAGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5594 | 1.1 | 0.000209 |
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Target: 5'- gUCAGCCUUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGAAUCGAAUCCAGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5490 | 1.1 | 0.000209 |
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Target: 5'- gUCAGCCUUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGAAUCGAAUCCAGGUCAGCAC- -5' |
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| 32385 | 5' | -52.1 | NC_007041.1 | + | 5386 | 1.1 | 0.000209 |
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Target: 5'- gUCAGCCUUAGCUUAGGUCCAGUCGUGg -3' miRNA: 3'- -AGUCGGAAUCGAAUCCAGGUCAGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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