Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32567 | 5' | -60.6 | NC_007151.1 | + | 126383 | 0.91 | 0.01918 |
Target: 5'- --cGUCGCCGCCGCCGCCGCCGUCGUg -3' miRNA: 3'- uaaCAGUGGCGGCGGUGGCGGUGGCG- -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 126870 | 0.73 | 0.310488 |
Target: 5'- -cUGUUGCUGUugcgaUGCCGCCGCCuCCGCc -3' miRNA: 3'- uaACAGUGGCG-----GCGGUGGCGGuGGCG- -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 127169 | 0.68 | 0.597248 |
Target: 5'- --cGUCGCCaGcCCGUgAUCGCCAUCGa -3' miRNA: 3'- uaaCAGUGG-C-GGCGgUGGCGGUGGCg -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 127171 | 0.84 | 0.055519 |
Target: 5'- --cGUCACCGUCGUCgacauaauccacaACCGCCACCGCa -3' miRNA: 3'- uaaCAGUGGCGGCGG-------------UGGCGGUGGCG- -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 134307 | 0.66 | 0.706354 |
Target: 5'- -cUGUCGgCGCCGCCGuaGUCGgCGa -3' miRNA: 3'- uaACAGUgGCGGCGGUggCGGUgGCg -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 142068 | 0.69 | 0.537371 |
Target: 5'- -cUGUCGCCGCCGCUgucaaagAUUGCCGauCUGUc -3' miRNA: 3'- uaACAGUGGCGGCGG-------UGGCGGU--GGCG- -5' |
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32567 | 5' | -60.6 | NC_007151.1 | + | 145829 | 0.66 | 0.666949 |
Target: 5'- ---uUUAUCGUgGCCugUGCCAUUGCa -3' miRNA: 3'- uaacAGUGGCGgCGGugGCGGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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