Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32567 | 5' | -60.6 | NC_007151.1 | + | 19465 | 0.67 | 0.617146 |
|
Target: 5'- ---cUCugCGCCuuGUCGCguaGCCGCCGCu -3' miRNA: 3'- uaacAGugGCGG--CGGUGg--CGGUGGCG- -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 49122 | 0.67 | 0.617146 |
|
Target: 5'- aAUUG--GCCGUCGUCAUCGaCCAUCGUg -3' miRNA: 3'- -UAACagUGGCGGCGGUGGC-GGUGGCG- -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 145829 | 0.66 | 0.666949 |
|
Target: 5'- ---uUUAUCGUgGCCugUGCCAUUGCa -3' miRNA: 3'- uaacAGUGGCGgCGGugGCGGUGGCG- -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 1494 | 0.66 | 0.686738 |
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Target: 5'- ----aUACCaCCGCCACCuucuuuGCCGCCGa -3' miRNA: 3'- uaacaGUGGcGGCGGUGG------CGGUGGCg -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 63342 | 0.66 | 0.696571 |
|
Target: 5'- aAUUGUUaaACCGCCGagACCGCuguuCAUUGCg -3' miRNA: 3'- -UAACAG--UGGCGGCggUGGCG----GUGGCG- -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 134307 | 0.66 | 0.706354 |
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Target: 5'- -cUGUCGgCGCCGCCGuaGUCGgCGa -3' miRNA: 3'- uaACAGUgGCGGCGGUggCGGUgGCg -5' |
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| 32567 | 5' | -60.6 | NC_007151.1 | + | 17153 | 0.66 | 0.725731 |
|
Target: 5'- -cUGUCGUCGUCGUCGUCGUCAUCGUc -3' miRNA: 3'- uaACAGUGGCGGCGGUGGCGGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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