Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
32578 | 3' | -57.2 | NC_007151.1 | + | 61574 | 0.66 | 0.887383 |
Target: 5'- aUGCuGCGGCuccCGGgAUUCCuGUGAUCGa -3' miRNA: 3'- gACG-CGCCG---GCUgUAGGGcUACUAGC- -5' |
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32578 | 3' | -57.2 | NC_007151.1 | + | 64644 | 0.66 | 0.878437 |
Target: 5'- aCUGUGCGGCauuggCGGCAacaccggcuggcucUCCUucUGAUCGg -3' miRNA: 3'- -GACGCGCCG-----GCUGU--------------AGGGcuACUAGC- -5' |
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32578 | 3' | -57.2 | NC_007151.1 | + | 91894 | 0.66 | 0.858717 |
Target: 5'- cCUGCcuccGCuGGagcgaCGACGcccgCCCGAUGAUCGu -3' miRNA: 3'- -GACG----CG-CCg----GCUGUa---GGGCUACUAGC- -5' |
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32578 | 3' | -57.2 | NC_007151.1 | + | 107678 | 0.67 | 0.843171 |
Target: 5'- --uCGCGGCCGuuGUCggcgaCGAUGAUUGa -3' miRNA: 3'- gacGCGCCGGCugUAGg----GCUACUAGC- -5' |
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32578 | 3' | -57.2 | NC_007151.1 | + | 64590 | 0.72 | 0.556946 |
Target: 5'- aUGCGCGGCCGGugccgauuacCGUUCCGAUccuuUCGg -3' miRNA: 3'- gACGCGCCGGCU----------GUAGGGCUAcu--AGC- -5' |
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32578 | 3' | -57.2 | NC_007151.1 | + | 118917 | 1.08 | 0.002869 |
Target: 5'- uCUGCGCGGCCGACAUCCCGAUGAUCGu -3' miRNA: 3'- -GACGCGCCGGCUGUAGGGCUACUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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