Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32580 | 5' | -43.1 | NC_007151.1 | + | 2077 | 0.71 | 0.999426 |
|
Target: 5'- ---cCGAAgGUAAAUUUGGCAAAGUGUc -3' miRNA: 3'- uguaGCUU-CGUUUAGACCGUUUUAUA- -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 15485 | 0.77 | 0.967128 |
|
Target: 5'- gGCaAUCGAAGCAAAUCUugcGGCAAcAAUAg -3' miRNA: 3'- -UG-UAGCUUCGUUUAGA---CCGUU-UUAUa -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 31572 | 0.67 | 0.999998 |
|
Target: 5'- aGCGUCGgcGcCAGAggCUGGgAGAAUAa -3' miRNA: 3'- -UGUAGCuuC-GUUUa-GACCgUUUUAUa -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 55502 | 0.68 | 0.999991 |
|
Target: 5'- --cUCGAAuCGAAUCUGGCAAu---- -3' miRNA: 3'- uguAGCUUcGUUUAGACCGUUuuaua -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 65900 | 0.7 | 0.999838 |
|
Target: 5'- cACAUCGGccGGCAGAUUUuGUAAAAUAUu -3' miRNA: 3'- -UGUAGCU--UCGUUUAGAcCGUUUUAUA- -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 110426 | 0.68 | 0.999981 |
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Target: 5'- uCAUCG--GCAuGUCUGGCGAAGa-- -3' miRNA: 3'- uGUAGCuuCGUuUAGACCGUUUUaua -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 124924 | 1.08 | 0.05194 |
|
Target: 5'- gACAUCGAAGCAAAUCUGGCAAAAUAUa -3' miRNA: 3'- -UGUAGCUUCGUUUAGACCGUUUUAUA- -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 137140 | 0.66 | 1 |
|
Target: 5'- uGCAUCGcaauAGCAAA---GGCGAAAUAc -3' miRNA: 3'- -UGUAGCu---UCGUUUagaCCGUUUUAUa -5' |
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| 32580 | 5' | -43.1 | NC_007151.1 | + | 146284 | 0.71 | 0.999547 |
|
Target: 5'- gAUAUCGAuggaaaaauccGCAAGUCUGGCAAc---- -3' miRNA: 3'- -UGUAGCUu----------CGUUUAGACCGUUuuaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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