Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32581 | 5' | -52.1 | NC_007151.1 | + | 97163 | 0.66 | 0.984857 |
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Target: 5'- gGUUAUaGACGAgUCUCUGCCAaguauUCGUUc -3' miRNA: 3'- gCGAUAcUUGCU-AGAGACGGU-----GGCAG- -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 50133 | 0.67 | 0.96759 |
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Target: 5'- gGCUAucacguccUGGGCGAUgUCUGCCAUgCGa- -3' miRNA: 3'- gCGAU--------ACUUGCUAgAGACGGUG-GCag -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 133707 | 0.68 | 0.944315 |
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Target: 5'- aCGCcaucgGUGAACGAauacugUCUCUGUCGuuGUa -3' miRNA: 3'- -GCGa----UACUUGCU------AGAGACGGUggCAg -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 70634 | 0.69 | 0.934606 |
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Target: 5'- uCGCUucaauCGAUCguuugCUGCCAUCGUUa -3' miRNA: 3'- -GCGAuacuuGCUAGa----GACGGUGGCAG- -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 95511 | 0.71 | 0.855483 |
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Target: 5'- uCGUUuUGAGCGca-UCcGCCACCGUCg -3' miRNA: 3'- -GCGAuACUUGCuagAGaCGGUGGCAG- -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 66608 | 0.75 | 0.653655 |
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Target: 5'- aCGCUAUGAGCGGUCUUUugaaCGCCGg- -3' miRNA: 3'- -GCGAUACUUGCUAGAGAcg--GUGGCag -5' |
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| 32581 | 5' | -52.1 | NC_007151.1 | + | 126312 | 1.11 | 0.004863 |
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Target: 5'- gCGCUAUGAACGAUCUCUGCCACCGUCa -3' miRNA: 3'- -GCGAUACUUGCUAGAGACGGUGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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