Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32584 | 3' | -44.5 | NC_007151.1 | + | 76835 | 0.66 | 0.999997 |
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Target: 5'- uUCGUU--UGAAUUAcAUCCGgGCAGu -3' miRNA: 3'- cAGCAAauAUUUGGUuUAGGCgCGUC- -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 147377 | 0.67 | 0.99999 |
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Target: 5'- cUCGUUguu--ACCuaGAAUCUGUGCAGu -3' miRNA: 3'- cAGCAAauauuUGG--UUUAGGCGCGUC- -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 88323 | 0.67 | 0.999986 |
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Target: 5'- -cUGUUUAUAAAuCCuuAUCgGCGCGa -3' miRNA: 3'- caGCAAAUAUUU-GGuuUAGgCGCGUc -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 73653 | 0.67 | 0.999986 |
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Target: 5'- aUCGUUUcuauacgggcgAUAGAUCAAgacauAUCCGUGUAGu -3' miRNA: 3'- cAGCAAA-----------UAUUUGGUU-----UAGGCGCGUC- -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 71087 | 0.67 | 0.999981 |
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Target: 5'- uGUCGUUcgacacgaaUAUAGAuCCAguguuguucucGAUCCGUGCAc -3' miRNA: 3'- -CAGCAA---------AUAUUU-GGU-----------UUAGGCGCGUc -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 70915 | 0.71 | 0.998974 |
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Target: 5'- --aGUggGUGAGCCGAAUCCGUGa-- -3' miRNA: 3'- cagCAaaUAUUUGGUUUAGGCGCguc -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 71000 | 0.74 | 0.990336 |
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Target: 5'- aGUCGUUcaac-ACUAGAUCCGCGCu- -3' miRNA: 3'- -CAGCAAauauuUGGUUUAGGCGCGuc -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 102740 | 0.78 | 0.926259 |
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Target: 5'- aUCGcUUUGUAGAugUCGAAUCCGCGCAu -3' miRNA: 3'- cAGC-AAAUAUUU--GGUUUAGGCGCGUc -5' |
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| 32584 | 3' | -44.5 | NC_007151.1 | + | 136820 | 1.1 | 0.034518 |
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Target: 5'- aGUCGUUUAUAAACCAAAUCCGCGCAGc -3' miRNA: 3'- -CAGCAAAUAUUUGGUUUAGGCGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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