miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3280 5' -43.2 NC_001543.1 + 6672 0.67 0.784967
Target:  5'- aCCCAcagguGCAGCggcaggagugccgGGUGUGGUUACAAUUc- -3'
miRNA:   3'- -GGGU-----UGUUG-------------UCACAUUAGUGUUGAaa -5'
3280 5' -43.2 NC_001543.1 + 1189 0.69 0.650361
Target:  5'- aCCCGACAACAGUGgaAAagACAAa--- -3'
miRNA:   3'- -GGGUUGUUGUCACa-UUagUGUUgaaa -5'
3280 5' -43.2 NC_001543.1 + 3711 0.72 0.45872
Target:  5'- cCCCAACAACuugccAGUGUGuAUgGCAACa-- -3'
miRNA:   3'- -GGGUUGUUG-----UCACAU-UAgUGUUGaaa -5'
3280 5' -43.2 NC_001543.1 + 1187 1.09 0.001534
Target:  5'- gCCCAACAACAGUGUAAUCACAACUUUc -3'
miRNA:   3'- -GGGUUGUUGUCACAUUAGUGUUGAAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.