Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33035 | 5' | -56.1 | NC_007497.1 | + | 35634 | 0.73 | 0.235705 |
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Target: 5'- -aCG-CGAucGCGCGCggcaugGGCCGGgGCa -3' miRNA: 3'- uaGUaGUUuuCGCGCGa-----CCGGCCgCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 35777 | 0.69 | 0.409967 |
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Target: 5'- gAUCAgCAAGgcGGUgGCGCUGuCCGGCGUa -3' miRNA: 3'- -UAGUaGUUU--UCG-CGCGACcGGCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 38242 | 0.7 | 0.338239 |
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Target: 5'- --aGUCGAcuaaggcgcucAGGCGCGCcacggucGCCGGCGCg -3' miRNA: 3'- uagUAGUU-----------UUCGCGCGac-----CGGCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 41483 | 0.68 | 0.459335 |
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Target: 5'- uUC-UCGAAGGCGCuCgGuGCCGGCGg -3' miRNA: 3'- uAGuAGUUUUCGCGcGaC-CGGCCGCg -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 41534 | 0.72 | 0.268984 |
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Target: 5'- uUCAaCGAGGGCGCGCUGuuGCgCGGCa- -3' miRNA: 3'- uAGUaGUUUUCGCGCGAC--CG-GCCGcg -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 43044 | 0.77 | 0.116425 |
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Target: 5'- -gCAUCG--AGCGCGCUgggcuucuauacaaGGCCGGCGUc -3' miRNA: 3'- uaGUAGUuuUCGCGCGA--------------CCGGCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 43168 | 0.67 | 0.522465 |
|
Target: 5'- -cCGUCcgugcuGGAGCGcCGCaaagagauUGcGCCGGCGCu -3' miRNA: 3'- uaGUAGu-----UUUCGC-GCG--------AC-CGGCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 43691 | 0.72 | 0.276079 |
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Target: 5'- gGUCAUCAuccugacaacgcAAAGCGacgagcCGCcGGCCGGUGUg -3' miRNA: 3'- -UAGUAGU------------UUUCGC------GCGaCCGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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