Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33041 | 3' | -52.1 | NC_007497.1 | + | 16703 | 0.72 | 0.486596 |
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Target: 5'- uGCG-CGAGUUGCCACGCUucUUCcgUGa -3' miRNA: 3'- -CGCuGCUCAAUGGUGCGGu-GAGuaGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 17332 | 0.73 | 0.455584 |
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Target: 5'- cGCGAggcuuuCGAGcgUGCUGCGCUGCUCGUCc -3' miRNA: 3'- -CGCU------GCUCa-AUGGUGCGGUGAGUAGc -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 1512 | 0.75 | 0.378519 |
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Target: 5'- uGCGAUGAGcu-CCGCGCCGgUCAcCGa -3' miRNA: 3'- -CGCUGCUCaauGGUGCGGUgAGUaGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 13170 | 0.77 | 0.266184 |
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Target: 5'- uCGACGuguuguucGUUAUCGCGCUGCUCAUCGu -3' miRNA: 3'- cGCUGCu-------CAAUGGUGCGGUGAGUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 4329 | 0.77 | 0.259287 |
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Target: 5'- gGCGACGca----CACGCCGCUCAUCGc -3' miRNA: 3'- -CGCUGCucaaugGUGCGGUGAGUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 11708 | 1.1 | 0.001528 |
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Target: 5'- uGCGACGAGUUACCACGCCACUCAUCGc -3' miRNA: 3'- -CGCUGCUCAAUGGUGCGGUGAGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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