Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33041 | 5' | -53.4 | NC_007497.1 | + | 19298 | 0.66 | 0.75369 |
Target: 5'- cCGCAguAUGCGc-AGCGGCcgucgaaCAUUUGGCg -3' miRNA: 3'- -GCGU--UACGCcaUCGUCGa------GUAGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 1125 | 0.66 | 0.7429 |
Target: 5'- uGCcGUGCucguGUGGCAagucguGCUguUCUGGCa -3' miRNA: 3'- gCGuUACGc---CAUCGU------CGAguAGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 9097 | 0.66 | 0.7429 |
Target: 5'- gCGCGGgcUGCGGU-GCAGgCcgggggAUCUGGCu -3' miRNA: 3'- -GCGUU--ACGCCAuCGUC-Gag----UAGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 16082 | 0.66 | 0.7429 |
Target: 5'- uCGCGc-GCGGUAGCccaaggccGGUUUAUC-GGCg -3' miRNA: 3'- -GCGUuaCGCCAUCG--------UCGAGUAGaCCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 14071 | 0.66 | 0.7429 |
Target: 5'- uGUA--GCGGgAGCAGUUgGUCaGGCa -3' miRNA: 3'- gCGUuaCGCCaUCGUCGAgUAGaCCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 13101 | 0.66 | 0.73199 |
Target: 5'- aCGCAcgGCaccGUcgAGCAGUUCAUgcgCUGGUu -3' miRNA: 3'- -GCGUuaCGc--CA--UCGUCGAGUA---GACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 30140 | 0.66 | 0.73199 |
Target: 5'- cCGCAcucgggaccGCGGUAGC-GC-CA-CUGGCa -3' miRNA: 3'- -GCGUua-------CGCCAUCGuCGaGUaGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 457 | 0.66 | 0.73199 |
Target: 5'- aGCAGUGCGGcgcGCGGUUaCGguuacgaaUGGCa -3' miRNA: 3'- gCGUUACGCCau-CGUCGA-GUag------ACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 3518 | 0.67 | 0.69754 |
Target: 5'- cCGCGGUGagggaCGGUGGCGGCUgGcaaacggaggaauUCUgauGGCg -3' miRNA: 3'- -GCGUUAC-----GCCAUCGUCGAgU-------------AGA---CCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 39487 | 0.67 | 0.664709 |
Target: 5'- aCGC--UGCGuGUuGCGGUUCAUCgcgaagcgGGCg -3' miRNA: 3'- -GCGuuACGC-CAuCGUCGAGUAGa-------CCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 1340 | 0.67 | 0.664709 |
Target: 5'- gGCAuuugAUGCaGU-GCGGUUCGcgCUGGCg -3' miRNA: 3'- gCGU----UACGcCAuCGUCGAGUa-GACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 16578 | 0.68 | 0.584855 |
Target: 5'- gCGCAGUGCGGUcaugucgucGGCA-UUCAUCcGGa -3' miRNA: 3'- -GCGUUACGCCA---------UCGUcGAGUAGaCCg -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 16854 | 0.69 | 0.573537 |
Target: 5'- gCGCuGAUGCGG--GCuGCUCGUCcgccGGCg -3' miRNA: 3'- -GCG-UUACGCCauCGuCGAGUAGa---CCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 14384 | 0.7 | 0.496224 |
Target: 5'- gCGCGAUGCaGagccgcgGGCcGCUCG-CUGGCg -3' miRNA: 3'- -GCGUUACGcCa------UCGuCGAGUaGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 35773 | 0.71 | 0.414162 |
Target: 5'- aGCAagGCGGUGGC-GCU-GUCcGGCg -3' miRNA: 3'- gCGUuaCGCCAUCGuCGAgUAGaCCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 32320 | 0.71 | 0.413189 |
Target: 5'- aGCGGUGCuGGUgcuggcgGGCAGUUCGg-UGGCg -3' miRNA: 3'- gCGUUACG-CCA-------UCGUCGAGUagACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 35015 | 0.72 | 0.408347 |
Target: 5'- aGCaAGUGCGGUcgcacaggucgcgacGGCccGCUCGaaUCUGGCg -3' miRNA: 3'- gCG-UUACGCCA---------------UCGu-CGAGU--AGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 39641 | 0.72 | 0.404498 |
Target: 5'- aGCGGUGCGGcGGCAGacaUCAcgUCggacGGCa -3' miRNA: 3'- gCGUUACGCCaUCGUCg--AGU--AGa---CCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 33704 | 0.72 | 0.376378 |
Target: 5'- aGCAgAUGgaGGUcgcgaAGCAGCUC-UCUGGCu -3' miRNA: 3'- gCGU-UACg-CCA-----UCGUCGAGuAGACCG- -5' |
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33041 | 5' | -53.4 | NC_007497.1 | + | 34636 | 0.72 | 0.376378 |
Target: 5'- gGCGGUcgccGCGGUGGCGcCUCGUUcGGCg -3' miRNA: 3'- gCGUUA----CGCCAUCGUcGAGUAGaCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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