miRNA: 3'- -GCGUuaCGCCauCGUCGAgUAGaCCG- -5'
miRNA: 3'- -GCGUuaCGCCaUCGUCGaGUAGaCCG- -5'
miRNA: 3'- gCGUUa---CGCCauCGUCGAGUAGa--CCG- -5'
miRNA: 3'- -GCGUUACGCCAUCGUCGAGUAGACCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33041 | 5' | -53.4 | NC_007497.1 | + | 14447 | 0.73 | 0.32422 |
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Target: 5'- cCGCAggGCGGgcGCGGUUgAUCaGGUg -3' miRNA: 3'- -GCGUuaCGCCauCGUCGAgUAGaCCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 27191 | 0.73 | 0.316068 |
|
Target: 5'- gCGCAagGCGG-GGCGGCgCAUC-GGCg -3' miRNA: 3'- -GCGUuaCGCCaUCGUCGaGUAGaCCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 21555 | 0.76 | 0.206312 |
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Target: 5'- gGCAGaggcGUGGgcGCAGCUCGUCgacGGCg -3' miRNA: 3'- gCGUUa---CGCCauCGUCGAGUAGa--CCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 11674 | 1.11 | 0.000692 |
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Target: 5'- cCGCAAUGCGGUAGCAGCUCAUCUGGCc -3' miRNA: 3'- -GCGUUACGCCAUCGUCGAGUAGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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