miRNA: 3'- -GCGUuaCGCCAUCG--------UCGAGUAGaCCG- -5'
miRNA: 3'- -GCGUU--ACGCCAuCGUC-Gag----UAGACCG- -5'
miRNA: 3'- gCGuUACGc---CAUCGU------CGAguAGACCG- -5'
miRNA: 3'- -GCGU--UACGCcaUCGUCGa------GUAGACCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33041 | 5' | -53.4 | NC_007497.1 | + | 16082 | 0.66 | 0.7429 |
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Target: 5'- uCGCGc-GCGGUAGCccaaggccGGUUUAUC-GGCg -3' miRNA: 3'- -GCGUuaCGCCAUCG--------UCGAGUAGaCCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 9097 | 0.66 | 0.7429 |
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Target: 5'- gCGCGGgcUGCGGU-GCAGgCcgggggAUCUGGCu -3' miRNA: 3'- -GCGUU--ACGCCAuCGUC-Gag----UAGACCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 1125 | 0.66 | 0.7429 |
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Target: 5'- uGCcGUGCucguGUGGCAagucguGCUguUCUGGCa -3' miRNA: 3'- gCGuUACGc---CAUCGU------CGAguAGACCG- -5' |
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| 33041 | 5' | -53.4 | NC_007497.1 | + | 19298 | 0.66 | 0.75369 |
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Target: 5'- cCGCAguAUGCGc-AGCGGCcgucgaaCAUUUGGCg -3' miRNA: 3'- -GCGU--UACGCcaUCGUCGa------GUAGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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