Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33047 | 3' | -53.9 | NC_007497.1 | + | 9803 | 0.72 | 0.359346 |
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Target: 5'- aGUAGAuaucGAuAACCuGcCGCAGCCGAUCg -3' miRNA: 3'- gCGUCU----CU-UUGGuCuGCGUCGGCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 14447 | 0.72 | 0.359346 |
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Target: 5'- cCGCAGGG----CGGGCGCGGuuGAUCa -3' miRNA: 3'- -GCGUCUCuuugGUCUGCGUCggCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 10545 | 0.71 | 0.414761 |
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Target: 5'- uGUcGAGcAGCCAGACgGCAGCgUGAUCg -3' miRNA: 3'- gCGuCUCuUUGGUCUG-CGUCG-GCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 16148 | 0.7 | 0.475121 |
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Target: 5'- gGCGGAuuGAGCCAGACGCGcCCGcgCc -3' miRNA: 3'- gCGUCUc-UUUGGUCUGCGUcGGCuaG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 3516 | 0.69 | 0.539552 |
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Target: 5'- gCGguGAGGGA-CGGugGCGGCUGGc- -3' miRNA: 3'- -GCguCUCUUUgGUCugCGUCGGCUag -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 35150 | 0.67 | 0.663328 |
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Target: 5'- gGCGGcGGAggUCGcGGCGCAGgCCGAg- -3' miRNA: 3'- gCGUC-UCUuuGGU-CUGCGUC-GGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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