Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33114 | 3' | -51.2 | NC_007497.1 | + | 5571 | 0.67 | 0.815101 |
Target: 5'- ---cGAAGCgcGUCGGcggcggccugcugaaUccggAGCCGAUGCGCg -3' miRNA: 3'- cucaCUUCG--UAGCU---------------A----UCGGCUACGCG- -5' |
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33114 | 3' | -51.2 | NC_007497.1 | + | 16742 | 0.66 | 0.830511 |
Target: 5'- cGAGUGcgaaAUcCGGcUGGCUGGUGCGCg -3' miRNA: 3'- -CUCACuucgUA-GCU-AUCGGCUACGCG- -5' |
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33114 | 3' | -51.2 | NC_007497.1 | + | 1573 | 0.66 | 0.84894 |
Target: 5'- aGGUGAucgccgcuGGUAUCGAUGGCgGcgGCc- -3' miRNA: 3'- cUCACU--------UCGUAGCUAUCGgCuaCGcg -5' |
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33114 | 3' | -51.2 | NC_007497.1 | + | 8680 | 0.66 | 0.84894 |
Target: 5'- -cGUGGgcuGGCGUCGAcGGCCGGcgGCu- -3' miRNA: 3'- cuCACU---UCGUAGCUaUCGGCUa-CGcg -5' |
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33114 | 3' | -51.2 | NC_007497.1 | + | 28452 | 0.66 | 0.866367 |
Target: 5'- cGAGguuGGCGUCGAgcAGCgCGuAUGCGUu -3' miRNA: 3'- -CUCacuUCGUAGCUa-UCG-GC-UACGCG- -5' |
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33114 | 3' | -51.2 | NC_007497.1 | + | 36153 | 0.66 | 0.874681 |
Target: 5'- -cGUGAAGCGgaaGcgGGCgGG-GCGCg -3' miRNA: 3'- cuCACUUCGUag-CuaUCGgCUaCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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