Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33169 | 5' | -55.4 | NC_007497.1 | + | 43208 | 0.67 | 0.592948 |
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Target: 5'- uGGGCGGaacuggCUCG-CAUGgGcGCACGCu- -3' miRNA: 3'- -CCCGCCa-----GAGCuGUAUgU-CGUGCGcu -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 40308 | 0.67 | 0.60398 |
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Target: 5'- aGGCgGGUCUgcCGACGUccgACGcGUugGCGAa -3' miRNA: 3'- cCCG-CCAGA--GCUGUA---UGU-CGugCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 23561 | 0.67 | 0.615036 |
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Target: 5'- cGGCGGgcgC-CGGCGUcguGCAccugaucagcGCGCGCGAg -3' miRNA: 3'- cCCGCCa--GaGCUGUA---UGU----------CGUGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 29452 | 0.67 | 0.626107 |
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Target: 5'- cGGUuuUCUCGACGU-CGGC-CGCGAc -3' miRNA: 3'- cCCGccAGAGCUGUAuGUCGuGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 1314 | 0.66 | 0.637183 |
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Target: 5'- uGGCGuaCUCGACGcaGCAGUACgGCGAa -3' miRNA: 3'- cCCGCcaGAGCUGUa-UGUCGUG-CGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 10949 | 0.66 | 0.648255 |
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Target: 5'- gGGGCGGUagcgCaGCGUuCGGUGCGCGu -3' miRNA: 3'- -CCCGCCAga--GcUGUAuGUCGUGCGCu -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 14764 | 0.66 | 0.659312 |
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Target: 5'- uGGUGGugaUCUCGuuCAUGCuucaccucGCGCGCGGa -3' miRNA: 3'- cCCGCC---AGAGCu-GUAUGu-------CGUGCGCU- -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 37120 | 0.66 | 0.684628 |
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Target: 5'- cGGCGGccgUCgacggcaucgugacgUCGACGUACAGCguucgggucagcgGCGCGc -3' miRNA: 3'- cCCGCC---AG---------------AGCUGUAUGUCG-------------UGCGCu -5' |
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| 33169 | 5' | -55.4 | NC_007497.1 | + | 34635 | 0.66 | 0.692286 |
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Target: 5'- cGGCGGUCgccgCGGuggcgccuCGUuCGGCGCGCu- -3' miRNA: 3'- cCCGCCAGa---GCU--------GUAuGUCGUGCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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