Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33270 | 5' | -53.3 | NC_007605.1 | + | 63632 | 0.71 | 0.825991 |
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Target: 5'- uCGUCGGGggGCUGGgccugCAGCCGuGCc -3' miRNA: 3'- -GCGGCCCuaCGAUUacaa-GUCGGU-CG- -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 57476 | 0.71 | 0.808449 |
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Target: 5'- aGCCGGGGcgGCUGAUGgcgUgGGCUgcuGCu -3' miRNA: 3'- gCGGCCCUa-CGAUUACa--AgUCGGu--CG- -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 66247 | 0.71 | 0.808449 |
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Target: 5'- cCGCCgaucugGGGGUGauggAGUGUaaGGCCAGCg -3' miRNA: 3'- -GCGG------CCCUACga--UUACAagUCGGUCG- -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 53688 | 0.72 | 0.780922 |
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Target: 5'- aGCCGcaGcgGCUAG-GUUCAGCCAGg -3' miRNA: 3'- gCGGCc-CuaCGAUUaCAAGUCGGUCg -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 75725 | 0.73 | 0.691951 |
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Target: 5'- gCGCCGGGGUGg-GGUGgacgUCAGCCuGa -3' miRNA: 3'- -GCGGCCCUACgaUUACa---AGUCGGuCg -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 76253 | 0.75 | 0.62988 |
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Target: 5'- uGaCCGGGGUGCUGc----CGGCCGGCg -3' miRNA: 3'- gC-GGCCCUACGAUuacaaGUCGGUCG- -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 2793 | 0.76 | 0.551527 |
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Target: 5'- gGCCGGGGUGCUGGcgucucauaaacagcUGUgggCAGUUGGCc -3' miRNA: 3'- gCGGCCCUACGAUU---------------ACAa--GUCGGUCG- -5' |
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| 33270 | 5' | -53.3 | NC_007605.1 | + | 5460 | 1.13 | 0.00312 |
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Target: 5'- uCGCCGGGAUGCUAAUGUUCAGCCAGCg -3' miRNA: 3'- -GCGGCCCUACGAUUACAAGUCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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