Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33271 | 5' | -57 | NC_007605.1 | + | 50686 | 0.67 | 0.822629 |
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Target: 5'- aGGCGGGUGGCggAgGGCCGaaucucCGCGg- -3' miRNA: 3'- -CUGCCCACCGa-UgUCGGUgu----GUGCag -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 50583 | 0.71 | 0.620395 |
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Target: 5'- cACGGG-GGCgcugGCGGCCgGCGCcCGUCc -3' miRNA: 3'- cUGCCCaCCGa---UGUCGG-UGUGuGCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 49369 | 0.66 | 0.862687 |
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Target: 5'- --aGGGUgGGCUuggGCAGCCGgcgugccauaACGCGUCu -3' miRNA: 3'- cugCCCA-CCGA---UGUCGGUg---------UGUGCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 40553 | 0.66 | 0.862687 |
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Target: 5'- aGCGGGUGGC-GCGgggucccuGCCugGCACcaggGUCc -3' miRNA: 3'- cUGCCCACCGaUGU--------CGGugUGUG----CAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 40085 | 0.66 | 0.88432 |
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Target: 5'- cGACcuGGGUGGgUGCGGuCCGCugGCc-- -3' miRNA: 3'- -CUG--CCCACCgAUGUC-GGUGugUGcag -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 36031 | 0.7 | 0.659972 |
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Target: 5'- --aGGGUGG--GCAGCCugggauaACACGCGUCc -3' miRNA: 3'- cugCCCACCgaUGUCGG-------UGUGUGCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 6857 | 1.09 | 0.002558 |
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Target: 5'- gGACGGGUGGCUACAGCCACACACGUCu -3' miRNA: 3'- -CUGCCCACCGAUGUCGGUGUGUGCAG- -5' |
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| 33271 | 5' | -57 | NC_007605.1 | + | 6659 | 0.7 | 0.701206 |
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Target: 5'- aGACGuGuGUGGCUGUAGCCAC-C-CGUCc -3' miRNA: 3'- -CUGC-C-CACCGAUGUCGGUGuGuGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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