Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33283 | 3' | -53.9 | NC_007605.1 | + | 157271 | 0.66 | 0.949063 |
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Target: 5'- aAGgUCUCCCAGcaUGCGGUugaucuGGCggcgcagGGa -3' miRNA: 3'- -UCgAGAGGGUC--ACGUCAuuu---CCGa------CC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 157444 | 0.68 | 0.918953 |
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Target: 5'- uGCUC-CCgCGGgccGCGGauGGGGCUGGg -3' miRNA: 3'- uCGAGaGG-GUCa--CGUCauUUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 159814 | 0.71 | 0.769747 |
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Target: 5'- aGGCgacgCUCCCGGgcaaacaggGCAG-AGAGGCgGGa -3' miRNA: 3'- -UCGa---GAGGGUCa--------CGUCaUUUCCGaCC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 159873 | 0.69 | 0.872754 |
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Target: 5'- cAGCUC-CUCGGgcgacGCGGUgGAGGGCUGa -3' miRNA: 3'- -UCGAGaGGGUCa----CGUCA-UUUCCGACc -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 163342 | 0.69 | 0.857486 |
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Target: 5'- aGGCUCccgagUCCgGGUGacugauGGUuGAGGCUGGg -3' miRNA: 3'- -UCGAG-----AGGgUCACg-----UCAuUUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 167303 | 0.66 | 0.964334 |
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Target: 5'- uGCUaC-CUguGUGguGUGuuaAGGGCUGGg -3' miRNA: 3'- uCGA-GaGGguCACguCAU---UUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 169729 | 0.68 | 0.913074 |
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Target: 5'- uAGCgccgCUCuGUGCGG-GGGGGCUGGg -3' miRNA: 3'- -UCGaga-GGGuCACGUCaUUUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 170266 | 0.68 | 0.913074 |
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Target: 5'- uAGCgccgCUCuGUGCGG-GGGGGCUGGg -3' miRNA: 3'- -UCGaga-GGGuCACGUCaUUUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 170789 | 0.68 | 0.913074 |
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Target: 5'- uAGCgccgCUCuGUGCGG-GGGGGCUGGg -3' miRNA: 3'- -UCGaga-GGGuCACGUCaUUUCCGACC- -5' |
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| 33283 | 3' | -53.9 | NC_007605.1 | + | 171326 | 0.68 | 0.913074 |
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Target: 5'- uAGCgccgCUCuGUGCGG-GGGGGCUGGg -3' miRNA: 3'- -UCGaga-GGGuCACGUCaUUUCCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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