Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33617 | 3' | -63.5 | NC_007605.1 | + | 61781 | 0.66 | 0.588331 |
Target: 5'- cAGUCCgGAGacugaGGCCUuuGuccguaaccuugaCCGUCCAc -3' miRNA: 3'- uUCAGGgCUCg----CCGGAggC-------------GGCAGGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 110691 | 0.66 | 0.579648 |
Target: 5'- ---cCCCGAGCugacccGGgC-CCGCCGUCCu -3' miRNA: 3'- uucaGGGCUCG------CCgGaGGCGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 4093 | 0.66 | 0.570033 |
Target: 5'- -uGcCCCGAGacCGGCUcgUCCGCC-UCCAa -3' miRNA: 3'- uuCaGGGCUC--GCCGG--AGGCGGcAGGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 33380 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 27242 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 24173 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 21104 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 18035 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 30311 | 0.66 | 0.560457 |
Target: 5'- uAGUcCCCGGGCuucaGGCCcCCuCCGUCCc -3' miRNA: 3'- uUCA-GGGCUCG----CCGGaGGcGGCAGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 109014 | 0.67 | 0.550926 |
Target: 5'- ---aCCCGgcGGCGGuCCUCCGCCa-CCAc -3' miRNA: 3'- uucaGGGC--UCGCC-GGAGGCGGcaGGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 110114 | 0.67 | 0.550926 |
Target: 5'- uGG-CCCGGGCGGCCUaCCugcgggcggagGCCGaggCCGu -3' miRNA: 3'- uUCaGGGCUCGCCGGA-GG-----------CGGCa--GGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 112388 | 0.67 | 0.550926 |
Target: 5'- --cUCUCG-GCGGCUgCCGCCG-CCAg -3' miRNA: 3'- uucAGGGCuCGCCGGaGGCGGCaGGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 156834 | 0.67 | 0.541445 |
Target: 5'- aAGGUCUgcaGGGUGGCaa-UGCCGUCCAg -3' miRNA: 3'- -UUCAGGg--CUCGCCGgagGCGGCAGGU- -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 13023 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 19161 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 34506 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 31437 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 28368 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 25299 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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33617 | 3' | -63.5 | NC_007605.1 | + | 16092 | 0.67 | 0.522654 |
Target: 5'- cAG-CCCGGGCGGCC-CCagagGCCGguUCCu -3' miRNA: 3'- uUCaGGGCUCGCCGGaGG----CGGC--AGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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