Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
342 | 3' | -52.7 | AC_000011.1 | + | 18694 | 0.66 | 0.710709 |
Target: 5'- -gUAGG-CGgUGCCGGAGUAGggUUUg -3' miRNA: 3'- cgAUCCaGCgGUGGCUUCGUCuuAGA- -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 22012 | 0.67 | 0.676482 |
Target: 5'- uGCUGGG-CGCCcugcaACUGAcGCGGAAgCUg -3' miRNA: 3'- -CGAUCCaGCGG-----UGGCUuCGUCUUaGA- -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 3100 | 0.67 | 0.653409 |
Target: 5'- --gGGGUC-CCGCCGAGGCAuguUCa -3' miRNA: 3'- cgaUCCAGcGGUGGCUUCGUcuuAGa -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 12996 | 0.67 | 0.653409 |
Target: 5'- uGCUGaG-CGCgUACUGggGCGGGAUCUu -3' miRNA: 3'- -CGAUcCaGCG-GUGGCuuCGUCUUAGA- -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 5236 | 0.68 | 0.595551 |
Target: 5'- gGCUGGG-CGCUugCGAGGguGcgcuUCa -3' miRNA: 3'- -CGAUCCaGCGGugGCUUCguCuu--AGa -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 10990 | 0.68 | 0.582891 |
Target: 5'- gGCUGGcgcgccugggggcGUCGUCGCCGGAGCGGc---- -3' miRNA: 3'- -CGAUC-------------CAGCGGUGGCUUCGUCuuaga -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 26842 | 0.69 | 0.572573 |
Target: 5'- cGCUGGGUCuGCgACCuGA-CGGAGUCUu -3' miRNA: 3'- -CGAUCCAG-CGgUGGcUUcGUCUUAGA- -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 3890 | 0.69 | 0.54981 |
Target: 5'- uGCUGGGUCaGCuCGCCcAGGCGcuGggUCa -3' miRNA: 3'- -CGAUCCAG-CG-GUGGcUUCGU--CuuAGa -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 10952 | 0.69 | 0.527334 |
Target: 5'- gGCUGGaaGUUGCUGCUGggGCGGGggCa -3' miRNA: 3'- -CGAUC--CAGCGGUGGCuuCGUCUuaGa -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 20078 | 0.74 | 0.281385 |
Target: 5'- cGC-AGGUCGUUGCCGAGggagcucuGCAGGAUCa -3' miRNA: 3'- -CGaUCCAGCGGUGGCUU--------CGUCUUAGa -5' |
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342 | 3' | -52.7 | AC_000011.1 | + | 1746 | 1.1 | 0.000915 |
Target: 5'- aGCUAGGUCGCCACCGAAGCAGAAUCUc -3' miRNA: 3'- -CGAUCCAGCGGUGGCUUCGUCUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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