miRNA: 3'- gUUUCCAU-UCGAG-------GCGCGgU-------------UUCCGG- -5'
miRNA: 3'- gUUUCCauUCGAGGCG--CGGUuucCGG- -5'
miRNA: 3'- guuUCCAuuCGAG-------GCGCGGuuuCCGG- -5'
miRNA: 3'- guuuCCAU--------UCG--AGGCGCgGU--UUCCGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 349 | 3' | -52.8 | AC_000011.1 | + | 15507 | 0.66 | 0.70092 |
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Target: 5'- aGAAGGUGcGGCUCgagaggaCGCGCgGcgcgacuaugcccAGGGCCu -3' miRNA: 3'- gUUUCCAU-UCGAG-------GCGCGgU-------------UUCCGG- -5' |
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| 349 | 3' | -52.8 | AC_000011.1 | + | 23326 | 0.66 | 0.703199 |
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Target: 5'- gGAAGG--GGCUCUGCcaGCCAuccGCCa -3' miRNA: 3'- gUUUCCauUCGAGGCG--CGGUuucCGG- -5' |
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| 349 | 3' | -52.8 | AC_000011.1 | + | 12874 | 0.66 | 0.713414 |
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Target: 5'- --uAGGUcuGCUCguccacgUGCGCCu--GGCCg -3' miRNA: 3'- guuUCCAuuCGAG-------GCGCGGuuuCCGG- -5' |
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| 349 | 3' | -52.8 | AC_000011.1 | + | 12254 | 0.66 | 0.725801 |
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Target: 5'- ---uGGUGgagaacaaGGCcaUCCGCGgCGacGAGGCCg -3' miRNA: 3'- guuuCCAU--------UCG--AGGCGCgGU--UUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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