Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
351 | 5' | -57.9 | AC_000011.1 | + | 21477 | 0.68 | 0.337716 |
Target: 5'- cCGCCugcgGGCUCUUCugcugcauguUCCugcACGCCUUCg -3' miRNA: 3'- aGCGGu---UCGAGGAG----------AGGu--UGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 9064 | 0.68 | 0.345282 |
Target: 5'- gUCGCCcAGCgCCUCcaaacguUCCAugGCCUc- -3' miRNA: 3'- -AGCGGuUCGaGGAG-------AGGUugCGGGag -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 31610 | 0.67 | 0.372266 |
Target: 5'- aUCGCCucuccuacGAGCUCCUgCagCAGCGCCa-- -3' miRNA: 3'- -AGCGG--------UUCGAGGA-GagGUUGCGGgag -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 23014 | 0.72 | 0.179542 |
Target: 5'- cCGCC-AGCUCCUCcucggccugUCUuuCGUCCUCg -3' miRNA: 3'- aGCGGuUCGAGGAG---------AGGuuGCGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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