Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
356 | 5' | -58.1 | AC_000011.1 | + | 21178 | 0.66 | 0.386987 |
Target: 5'- cUCCuGCucGCCGGaGCCCGc-GGCCa- -3' miRNA: 3'- -AGGuCGuuCGGCC-CGGGUuuCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 7566 | 0.66 | 0.396239 |
Target: 5'- cUCgAGC-AGCaCGGGCUCAgcGACCc- -3' miRNA: 3'- -AGgUCGuUCG-GCCCGGGUuuCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 11493 | 0.66 | 0.396239 |
Target: 5'- aCCgAGCccgaGGGCCGcuGGCUCcuGGACCUGg -3' miRNA: 3'- aGG-UCG----UUCGGC--CCGGGuuUCUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 12851 | 0.66 | 0.405633 |
Target: 5'- cCCAcGUGAGCCGcGCCCu-GGGCCa- -3' miRNA: 3'- aGGU-CGUUCGGCcCGGGuuUCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 12228 | 0.66 | 0.415165 |
Target: 5'- cUCCAGgauGGCCGaGaGCCgGuuGGCCUGg -3' miRNA: 3'- -AGGUCgu-UCGGC-C-CGGgUuuCUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 21225 | 0.66 | 0.434635 |
Target: 5'- cCCAGgAAGuaGGGCCCGcAGcCCa- -3' miRNA: 3'- aGGUCgUUCggCCCGGGUuUCuGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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