Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3579 | 5' | -60 | NC_001650.1 | + | 24340 | 0.71 | 0.483871 |
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Target: 5'- -uGGGGUCCCCGUCCaGCCUGaCGGGg -3' miRNA: 3'- cuCCCUGGGGGCAGG-CGGAUaGUCUg -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 83739 | 0.72 | 0.464851 |
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Target: 5'- -cGGGGCgCCCCGUgccugcaccucucCCaCCUAUCGGGCg -3' miRNA: 3'- cuCCCUG-GGGGCA-------------GGcGGAUAGUCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 101880 | 0.72 | 0.430602 |
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Target: 5'- aAGGGGCUCaCGUCCGCaaUGUCGGACc -3' miRNA: 3'- cUCCCUGGGgGCAGGCGg-AUAGUCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 171725 | 0.75 | 0.300315 |
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Target: 5'- cGGGGGGCCUCCGUgUGUCUGaUAGGCg -3' miRNA: 3'- -CUCCCUGGGGGCAgGCGGAUaGUCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 5018 | 0.75 | 0.300315 |
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Target: 5'- cGGGGGGCCUCCGUgUGUCUGaUAGGCg -3' miRNA: 3'- -CUCCCUGGGGGCAgGCGGAUaGUCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 177945 | 0.82 | 0.104033 |
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Target: 5'- gGAGGGGuCCUCCGUCCGCCUGUaaGGACc -3' miRNA: 3'- -CUCCCU-GGGGGCAGGCGGAUAg-UCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 11238 | 0.82 | 0.104033 |
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Target: 5'- gGAGGGGuCCUCCGUCCGCCUGUaaGGACc -3' miRNA: 3'- -CUCCCU-GGGGGCAGGCGGAUAg-UCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 171951 | 1.09 | 0.001509 |
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Target: 5'- gGAGGGACCCCCGUCCGCCUAUCAGACa -3' miRNA: 3'- -CUCCCUGGGGGCAGGCGGAUAGUCUG- -5' |
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| 3579 | 5' | -60 | NC_001650.1 | + | 5244 | 1.09 | 0.001509 |
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Target: 5'- gGAGGGACCCCCGUCCGCCUAUCAGACa -3' miRNA: 3'- -CUCCCUGGGGGCAGGCGGAUAGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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