Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
360 | 5' | -62.5 | AC_000011.1 | + | 34685 | 0.66 | 0.261149 |
Target: 5'- cGCCGG-CGGC-GG-UCU-CGGCGCUu -3' miRNA: 3'- aCGGCCuGCCGaCCgAGAcGUCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 15771 | 0.66 | 0.261149 |
Target: 5'- aGCCGG-CGGC-GGCgcaUCgcccgGCGGCaCCg -3' miRNA: 3'- aCGGCCuGCCGaCCG---AGa----CGUCGcGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 11138 | 0.66 | 0.254593 |
Target: 5'- cGCgGGGCGGgaG---CUGCGGCGCg -3' miRNA: 3'- aCGgCCUGCCgaCcgaGACGUCGCGg -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 8505 | 0.66 | 0.248174 |
Target: 5'- gGCagGGGCGGCaggGGCacguCGGCGCCg -3' miRNA: 3'- aCGg-CCUGCCGa--CCGagacGUCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 23263 | 0.66 | 0.244387 |
Target: 5'- uUGgCGGAUGGCUGGCagagccccuuccgcgUUcgggGguGCGCUc -3' miRNA: 3'- -ACgGCCUGCCGACCG---------------AGa---CguCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 21457 | 0.66 | 0.241889 |
Target: 5'- -cUCGG-UGGCcagGGCgCUGCGGCGCa -3' miRNA: 3'- acGGCCuGCCGa--CCGaGACGUCGCGg -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 16007 | 0.66 | 0.241889 |
Target: 5'- gGCgGGACauGCUGGCgauggccgCUGCcGcCGCCg -3' miRNA: 3'- aCGgCCUGc-CGACCGa-------GACGuC-GCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 22627 | 0.66 | 0.241889 |
Target: 5'- cUGCaCGGGugcaccgaGGCcgagGGCaagCUGCGGUGCCu -3' miRNA: 3'- -ACG-GCCUg-------CCGa---CCGa--GACGUCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 15881 | 0.67 | 0.223827 |
Target: 5'- gGCCaGACGcGC-GGCUUcagGCgccAGCGCCg -3' miRNA: 3'- aCGGcCUGC-CGaCCGAGa--CG---UCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 15927 | 0.67 | 0.206924 |
Target: 5'- cGCgCGGccACGGC-GGCg--GCAGCgGCCa -3' miRNA: 3'- aCG-GCC--UGCCGaCCGagaCGUCG-CGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 25755 | 0.67 | 0.206924 |
Target: 5'- cGCCGaggacGACGGuCUGGCg--GCGGCuGCUu -3' miRNA: 3'- aCGGC-----CUGCC-GACCGagaCGUCG-CGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 17354 | 0.68 | 0.196278 |
Target: 5'- -uCCGuGGCGGUgcaGGcCUCU-CAGCGCCa -3' miRNA: 3'- acGGC-CUGCCGa--CC-GAGAcGUCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 15831 | 0.68 | 0.196277 |
Target: 5'- cGCCGGGCGaugcgccgccGCcGGCUCU-UGGCGCg -3' miRNA: 3'- aCGGCCUGC----------CGaCCGAGAcGUCGCGg -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 12219 | 0.68 | 0.196277 |
Target: 5'- gGCCGaGAgcCGGUUGGC-CUGgGGCuGCUg -3' miRNA: 3'- aCGGC-CU--GCCGACCGaGACgUCG-CGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 34268 | 0.68 | 0.181206 |
Target: 5'- gGCgGGGCGGUgcUGGCcCUGUGGUuCCg -3' miRNA: 3'- aCGgCCUGCCG--ACCGaGACGUCGcGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 34730 | 0.68 | 0.176415 |
Target: 5'- cGCCGc-CGGCgaucGGUg-UGCAGCGCCc -3' miRNA: 3'- aCGGCcuGCCGa---CCGagACGUCGCGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 10143 | 0.69 | 0.162711 |
Target: 5'- gUGCgGcGGCGGCUGGCg--GUAGagcgGCCa -3' miRNA: 3'- -ACGgC-CUGCCGACCGagaCGUCg---CGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 3585 | 0.69 | 0.15836 |
Target: 5'- cGgUGGACGGCcGGCcCgUGCAGC-CCg -3' miRNA: 3'- aCgGCCUGCCGaCCGaG-ACGUCGcGG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 17092 | 0.69 | 0.149973 |
Target: 5'- cGCaccaGGGCGGCaggGGUggUUGCAGCGgCg -3' miRNA: 3'- aCGg---CCUGCCGa--CCGa-GACGUCGCgG- -5' |
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360 | 5' | -62.5 | AC_000011.1 | + | 11551 | 0.69 | 0.149973 |
Target: 5'- aGCCaG-CGGCccucgGGCUCggugauauucaGCAGCGCCu -3' miRNA: 3'- aCGGcCuGCCGa----CCGAGa----------CGUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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