Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 360 | 5' | -62.5 | AC_000011.1 | + | 8409 | 0.7 | 0.127184 |
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Target: 5'- -cUUGGGCGGCUGGggCgacggggGCGGUGCCu -3' miRNA: 3'- acGGCCUGCCGACCgaGa------CGUCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 10967 | 0.71 | 0.115093 |
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Target: 5'- gGCCGcGGCGGUcgUGGCUggaaguugCUGCuggggcgggggcagaAGCGCCg -3' miRNA: 3'- aCGGC-CUGCCG--ACCGA--------GACG---------------UCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 23508 | 0.71 | 0.11287 |
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Target: 5'- gGCUGGGCGGCggGGCgguuaagcuuucauUCUGCuGCuGCUg -3' miRNA: 3'- aCGGCCUGCCGa-CCG--------------AGACGuCG-CGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 11944 | 0.71 | 0.107637 |
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Target: 5'- gGCCcGGCGGCUGGg-CUGCcAGUGCa -3' miRNA: 3'- aCGGcCUGCCGACCgaGACG-UCGCGg -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 8473 | 0.74 | 0.066514 |
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Target: 5'- cGCCGGGCGGCaggGGCggCU-CGGgGCCc -3' miRNA: 3'- aCGGCCUGCCGa--CCGa-GAcGUCgCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 12113 | 1.11 | 0.000065 |
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Target: 5'- aUGCCGGACGGCUGGCUCUGCAGCGCCg -3' miRNA: 3'- -ACGGCCUGCCGACCGAGACGUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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