Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 360 | 5' | -62.5 | AC_000011.1 | + | 21457 | 0.66 | 0.241889 |
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Target: 5'- -cUCGG-UGGCcagGGCgCUGCGGCGCa -3' miRNA: 3'- acGGCCuGCCGa--CCGaGACGUCGCGg -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 23263 | 0.66 | 0.244387 |
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Target: 5'- uUGgCGGAUGGCUGGCagagccccuuccgcgUUcgggGguGCGCUc -3' miRNA: 3'- -ACgGCCUGCCGACCG---------------AGa---CguCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 8505 | 0.66 | 0.248174 |
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Target: 5'- gGCagGGGCGGCaggGGCacguCGGCGCCg -3' miRNA: 3'- aCGg-CCUGCCGa--CCGagacGUCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 11138 | 0.66 | 0.254593 |
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Target: 5'- cGCgGGGCGGgaG---CUGCGGCGCg -3' miRNA: 3'- aCGgCCUGCCgaCcgaGACGUCGCGg -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 34685 | 0.66 | 0.261149 |
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Target: 5'- cGCCGG-CGGC-GG-UCU-CGGCGCUu -3' miRNA: 3'- aCGGCCuGCCGaCCgAGAcGUCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 15771 | 0.66 | 0.261149 |
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Target: 5'- aGCCGG-CGGC-GGCgcaUCgcccgGCGGCaCCg -3' miRNA: 3'- aCGGCCuGCCGaCCG---AGa----CGUCGcGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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