Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 360 | 5' | -62.5 | AC_000011.1 | + | 23263 | 0.66 | 0.244387 |
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Target: 5'- uUGgCGGAUGGCUGGCagagccccuuccgcgUUcgggGguGCGCUc -3' miRNA: 3'- -ACgGCCUGCCGACCG---------------AGa---CguCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 23508 | 0.71 | 0.11287 |
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Target: 5'- gGCUGGGCGGCggGGCgguuaagcuuucauUCUGCuGCuGCUg -3' miRNA: 3'- aCGGCCUGCCGa-CCG--------------AGACGuCG-CGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 25755 | 0.67 | 0.206924 |
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Target: 5'- cGCCGaggacGACGGuCUGGCg--GCGGCuGCUu -3' miRNA: 3'- aCGGC-----CUGCC-GACCGagaCGUCG-CGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 34268 | 0.68 | 0.181206 |
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Target: 5'- gGCgGGGCGGUgcUGGCcCUGUGGUuCCg -3' miRNA: 3'- aCGgCCUGCCG--ACCGaGACGUCGcGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 34685 | 0.66 | 0.261149 |
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Target: 5'- cGCCGG-CGGC-GG-UCU-CGGCGCUu -3' miRNA: 3'- aCGGCCuGCCGaCCgAGAcGUCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 34730 | 0.68 | 0.176415 |
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Target: 5'- cGCCGc-CGGCgaucGGUg-UGCAGCGCCc -3' miRNA: 3'- aCGGCcuGCCGa---CCGagACGUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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