Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3603 | 3' | -53.3 | NC_001650.1 | + | 94133 | 0.66 | 0.967123 |
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Target: 5'- uCGCgCCACCCUGguucaGGgACAGggucucGUGCa -3' miRNA: 3'- -GCG-GGUGGGACa----UCgUGUCaua---CAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 76459 | 0.66 | 0.967123 |
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Target: 5'- cCGagCCGCCCuUGUgcuuGGCGCAGUAggacacgGUGCc -3' miRNA: 3'- -GCg-GGUGGG-ACA----UCGUGUCAUa------CAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 52843 | 0.66 | 0.970186 |
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Target: 5'- uGCCCGCCCUGggagcgGGCGCGc------- -3' miRNA: 3'- gCGGGUGGGACa-----UCGUGUcauacaug -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 22122 | 0.66 | 0.97304 |
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Target: 5'- aGCUCAgCUgGUuGCACAGcAUGUACg -3' miRNA: 3'- gCGGGUgGGaCAuCGUGUCaUACAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 36089 | 0.66 | 0.978146 |
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Target: 5'- cCGCCCGCCCcgcgugaaacuccagGUcccccgcGGCGCAGUc-GUACu -3' miRNA: 3'- -GCGGGUGGGa--------------CA-------UCGUGUCAuaCAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 62927 | 0.66 | 0.979528 |
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Target: 5'- gCGCCCACCCUuGUccuuuuucuGGCACAccuccucaucGUACg -3' miRNA: 3'- -GCGGGUGGGA-CA---------UCGUGUcaua------CAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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