Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 369 | 3' | -53 | AC_000011.1 | + | 21228 | 0.74 | 0.294721 |
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Target: 5'- gUGCCCAGGAAGuAGGgCCCGCAgcccaggucgcGGAUg -3' miRNA: 3'- gGCGGGUUCUUC-UUUgGGGCGU-----------UCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 7294 | 0.74 | 0.287094 |
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Target: 5'- aUCGUUgAAGAGGAucuuGCCCgCGCGGGGCa -3' miRNA: 3'- -GGCGGgUUCUUCUu---UGGG-GCGUUCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 8454 | 0.74 | 0.272309 |
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Target: 5'- gCCGCCCAAGAAGAAA---CGgGGGACg -3' miRNA: 3'- -GGCGGGUUCUUCUUUgggGCgUUCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 11107 | 0.75 | 0.256758 |
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Target: 5'- -aGCCCGAGGAGAugcgcgcGGCCCgguuccaCGCGGGGCg -3' miRNA: 3'- ggCGGGUUCUUCU-------UUGGG-------GCGUUCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 34227 | 0.75 | 0.251286 |
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Target: 5'- cCCGCCCGccaugcagcGAAGAGACCCCGgGucccGGCa -3' miRNA: 3'- -GGCGGGUu--------CUUCUUUGGGGCgUu---CUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 22320 | 0.77 | 0.185456 |
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Target: 5'- uCC-CUCGGuGAAGaAGACCCCGCAGGACu -3' miRNA: 3'- -GGcGGGUU-CUUC-UUUGGGGCGUUCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 3593 | 0.84 | 0.06242 |
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Target: 5'- cCCGCCCAgagGGGAGAcGCCCCGUcAGAUa -3' miRNA: 3'- -GGCGGGU---UCUUCUuUGGGGCGuUCUG- -5' |
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| 369 | 3' | -53 | AC_000011.1 | + | 23147 | 1.11 | 0.000758 |
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Target: 5'- gCCGCCCAAGAAGAAACCCCGCAAGACc -3' miRNA: 3'- -GGCGGGUUCUUCUUUGGGGCGUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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