Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 374 | 3' | -61.5 | AC_000011.1 | + | 15852 | 0.7 | 0.143716 |
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Target: 5'- -aUGGCGgGGGUGCUCCGGUgCCGCc -3' miRNA: 3'- ucGCCGCgUCCGUGAGGUCGaGGUGc -5' |
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| 374 | 3' | -61.5 | AC_000011.1 | + | 34264 | 0.72 | 0.105696 |
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Target: 5'- gGGCGGUGCuGGCcCUgUGGUUCCGCa -3' miRNA: 3'- -UCGCCGCGuCCGuGAgGUCGAGGUGc -5' |
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| 374 | 3' | -61.5 | AC_000011.1 | + | 11608 | 0.73 | 0.081837 |
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Target: 5'- cAGCGGCaGCccGCGCUCCuGCaCCACGa -3' miRNA: 3'- -UCGCCG-CGucCGUGAGGuCGaGGUGC- -5' |
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| 374 | 3' | -61.5 | AC_000011.1 | + | 15576 | 0.76 | 0.047256 |
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Target: 5'- cGCGuGCGCGGGCACuUCCGcGCUcCCugGg -3' miRNA: 3'- uCGC-CGCGUCCGUG-AGGU-CGA-GGugC- -5' |
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| 374 | 3' | -61.5 | AC_000011.1 | + | 17358 | 0.8 | 0.025524 |
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Target: 5'- uGGCGGUGCAGGC-CUCuCAGCgCCACu -3' miRNA: 3'- -UCGCCGCGUCCGuGAG-GUCGaGGUGc -5' |
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| 374 | 3' | -61.5 | AC_000011.1 | + | 24486 | 1.09 | 0.000131 |
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Target: 5'- aAGCGGCGCAGGCACUCCAGCUCCACGg -3' miRNA: 3'- -UCGCCGCGUCCGUGAGGUCGAGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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