Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 414 | 5' | -57.1 | AC_000011.1 | + | 23069 | 0.73 | 0.169859 |
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Target: 5'- -gGCCGAgGAGGAGCuGGCGGCcGUGGg -3' miRNA: 3'- ggCGGCUgCUCUUCG-UCGUCGuCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 11899 | 0.74 | 0.15621 |
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Target: 5'- gCCGCCgGGCcuuGGAGGCGGCGGCAGgAc -3' miRNA: 3'- -GGCGG-CUGc--UCUUCGUCGUCGUCgUc -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 8456 | 0.74 | 0.151886 |
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Target: 5'- gCGgCGGCGAGGacgcgcgccgGGCGGCAGgGGCGGc -3' miRNA: 3'- gGCgGCUGCUCU----------UCGUCGUCgUCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 3734 | 0.77 | 0.096093 |
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Target: 5'- gCGCUGGCGgcAGAuGCAGCAGCuGCGGc -3' miRNA: 3'- gGCGGCUGC--UCUuCGUCGUCGuCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 15907 | 0.78 | 0.073865 |
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Target: 5'- gCGCCGGCaggacccgGAGAcgcgcggccacGGCGGCGGCAGCGGc -3' miRNA: 3'- gGCGGCUG--------CUCU-----------UCGUCGUCGUCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 2070 | 0.79 | 0.067618 |
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Target: 5'- aCGCCaACGucgccAGCAGCAGCAGCAGg -3' miRNA: 3'- gGCGGcUGCucu--UCGUCGUCGUCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 12788 | 0.8 | 0.056618 |
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Target: 5'- gCCGCUGucCGuGAagggggccaccAGCAGCAGCAGCAGg -3' miRNA: 3'- -GGCGGCu-GCuCU-----------UCGUCGUCGUCGUC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 10130 | 0.8 | 0.054961 |
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Target: 5'- -aGCCGAUGAGGAaguGCGGCGGCGGCuGg -3' miRNA: 3'- ggCGGCUGCUCUU---CGUCGUCGUCGuC- -5' |
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| 414 | 5' | -57.1 | AC_000011.1 | + | 23415 | 1.11 | 0.000253 |
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Target: 5'- aCCGCCGACGAGAAGCAGCAGCAGCAGa -3' miRNA: 3'- -GGCGGCUGCUCUUCGUCGUCGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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