Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4147 | 3' | -53.4 | NC_001664.1 | + | 3507 | 0.68 | 0.913149 |
Target: 5'- gUCgCCGUCAcgguAGUCGuggcGGAgCACCCGGGg -3' miRNA: 3'- -AG-GGCGGUu---UCAGU----UCUgGUGGGUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 154589 | 0.68 | 0.913149 |
Target: 5'- gUCgCCGUCAcgguAGUCGuggcGGAgCACCCGGGg -3' miRNA: 3'- -AG-GGCGGUu---UCAGU----UCUgGUGGGUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 1111 | 0.68 | 0.913149 |
Target: 5'- cUCCGCCAcuG-CGAGGCCAUCUAu- -3' miRNA: 3'- aGGGCGGUuuCaGUUCUGGUGGGUuc -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 152193 | 0.68 | 0.913149 |
Target: 5'- cUCCGCCAcuG-CGAGGCCAUCUAu- -3' miRNA: 3'- aGGGCGGUuuCaGUUCUGGUGGGUuc -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 4067 | 0.69 | 0.899996 |
Target: 5'- gUCCCGCCucaugggcggaacAAGG--GAGACCGCCCu-- -3' miRNA: 3'- -AGGGCGG-------------UUUCagUUCUGGUGGGuuc -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 155149 | 0.69 | 0.899996 |
Target: 5'- gUCCCGCCucaugggcggaacAAGG--GAGACCGCCCu-- -3' miRNA: 3'- -AGGGCGG-------------UUUCagUUCUGGUGGGuuc -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 134307 | 0.69 | 0.8801 |
Target: 5'- -gCCGCUgauGGAGuucaaguuaUCAGGGCCGCCCAGa -3' miRNA: 3'- agGGCGG---UUUC---------AGUUCUGGUGGGUUc -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 146549 | 0.74 | 0.627599 |
Target: 5'- uUCCgGCCAAGGUgAGGAaaacggagguuCCugCCAAGg -3' miRNA: 3'- -AGGgCGGUUUCAgUUCU-----------GGugGGUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 151003 | 0.77 | 0.485526 |
Target: 5'- cCCCGCggcgCGAGGUC-AGACCGCgCCAGGg -3' miRNA: 3'- aGGGCG----GUUUCAGuUCUGGUG-GGUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 10562 | 0.77 | 0.482626 |
Target: 5'- aUCCCGCCGccgaugaaagagcaGAGUCucguucuuGGGCCACCuCAGGg -3' miRNA: 3'- -AGGGCGGU--------------UUCAGu-------UCUGGUGG-GUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 6645 | 0.95 | 0.039526 |
Target: 5'- gUCCCGCCAAAGUCAAGA-CACCCAAGc -3' miRNA: 3'- -AGGGCGGUUUCAGUUCUgGUGGGUUC- -5' |
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4147 | 3' | -53.4 | NC_001664.1 | + | 157727 | 1.09 | 0.005391 |
Target: 5'- gUCCCGCCAAAGUCAAGACCACCCAAGc -3' miRNA: 3'- -AGGGCGGUUUCAGUUCUGGUGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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