Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4159 | 3' | -46.2 | NC_001664.1 | + | 156139 | 0.69 | 0.998421 |
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Target: 5'- -cACGGuAGGUGGAucAGUUCUccgcgAUGCCGGc -3' miRNA: 3'- aaUGCU-UCCAUCU--UUAAGA-----UGCGGCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 154541 | 0.67 | 0.999711 |
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Target: 5'- -aGCGggGGUGGGAGggaagguggaUGgGCCGGc -3' miRNA: 3'- aaUGCuuCCAUCUUUaag-------AUgCGGCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 150094 | 1.08 | 0.028964 |
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Target: 5'- uUUACGAAGGUAGAAAUUCUACGCCGAg -3' miRNA: 3'- -AAUGCUUCCAUCUUUAAGAUGCGGCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 118844 | 0.66 | 0.999927 |
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Target: 5'- -gACGggGG-GGAAAUUCUAUGa-GAa -3' miRNA: 3'- aaUGCuuCCaUCUUUAAGAUGCggCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 44222 | 0.69 | 0.998703 |
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Target: 5'- gUUGCGAGGGgagUAGGAGUUUgcagGCGUgGAu -3' miRNA: 3'- -AAUGCUUCC---AUCUUUAAGa---UGCGgCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 11696 | 0.68 | 0.999443 |
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Target: 5'- -aACGAGGGuUAGAcAUUCgugaagcGCGCCGc -3' miRNA: 3'- aaUGCUUCC-AUCUuUAAGa------UGCGGCu -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 10632 | 0.68 | 0.999649 |
|
Target: 5'- -gACGAAGGUcGGAuucucUCUACgugGCCGAc -3' miRNA: 3'- aaUGCUUCCA-UCUuua--AGAUG---CGGCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 8770 | 0.66 | 0.99997 |
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Target: 5'- -cACGAAcuacGGUAGAAGcUUCgcaagACGCCa- -3' miRNA: 3'- aaUGCUU----CCAUCUUU-AAGa----UGCGGcu -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 5057 | 0.69 | 0.998421 |
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Target: 5'- -cACGGuAGGUGGAucAGUUCUccgcgAUGCCGGc -3' miRNA: 3'- aaUGCU-UCCAUCU--UUAAGA-----UGCGGCU- -5' |
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| 4159 | 3' | -46.2 | NC_001664.1 | + | 3460 | 0.67 | 0.999711 |
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Target: 5'- -aGCGggGGUGGGAGggaagguggaUGgGCCGGc -3' miRNA: 3'- aaUGCuuCCAUCUUUaag-------AUgCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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