Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 416 | 3' | -58.1 | AC_000011.1 | + | 23113 | 0.68 | 0.359331 |
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Target: 5'- gUGGUCcugcAGGACGUCAGCcaggacaGCGAGGa- -3' miRNA: 3'- -ACUAGc---UCCUGCAGUCGc------CGCUCCcg -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 23186 | 0.69 | 0.293163 |
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Target: 5'- uUGGUcCGAGGccACG-CGGCGGUagguaugucucuucGGGGGCa -3' miRNA: 3'- -ACUA-GCUCC--UGCaGUCGCCG--------------CUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 23464 | 0.67 | 0.37676 |
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Target: 5'- --uUCu----CGUCGGCGGUGGGGGCa -3' miRNA: 3'- acuAGcuccuGCAGUCGCCGCUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 23517 | 0.68 | 0.350831 |
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Target: 5'- aGGUgGcGGGGC-UgGGCGGCG-GGGCg -3' miRNA: 3'- aCUAgC-UCCUGcAgUCGCCGCuCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 24030 | 0.69 | 0.288039 |
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Target: 5'- aGGUUGAaaaGGGCGUCGGCG-CGGGuGCg -3' miRNA: 3'- aCUAGCU---CCUGCAGUCGCcGCUCcCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 25026 | 0.72 | 0.205359 |
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Target: 5'- --cUCGcGGAagGUCAGCGGCacgaaguccGAGGGCa -3' miRNA: 3'- acuAGCuCCUg-CAGUCGCCG---------CUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 25060 | 1.1 | 0.000266 |
|
Target: 5'- gUGAUCGAGGACGUCAGCGGCGAGGGCc -3' miRNA: 3'- -ACUAGCUCCUGCAGUCGCCGCUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 25753 | 0.66 | 0.442069 |
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Target: 5'- ---cCGAGGACGaCGGUcuGGCGGcGGCu -3' miRNA: 3'- acuaGCUCCUGCaGUCG--CCGCUcCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 26653 | 0.7 | 0.246994 |
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Target: 5'- gUGAUUGcGGGGCGU-GGCGGUGAGcGCu -3' miRNA: 3'- -ACUAGC-UCCUGCAgUCGCCGCUCcCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 36427 | 0.69 | 0.302866 |
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Target: 5'- aGA-CGGGcGAcCGUUAGgGGCG-GGGCg -3' miRNA: 3'- aCUaGCUC-CU-GCAGUCgCCGCuCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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