Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4164 | 3' | -49.4 | NC_001664.1 | + | 5830 | 1.12 | 0.007405 |
Target: 5'- aGAACAUCUGCACACGCAAAGGCAUCCc -3' miRNA: 3'- -CUUGUAGACGUGUGCGUUUCCGUAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 156912 | 1.12 | 0.007405 |
Target: 5'- aGAACAUCUGCACACGCAAAGGCAUCCc -3' miRNA: 3'- -CUUGUAGACGUGUGCGUUUCCGUAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 98969 | 0.81 | 0.502199 |
Target: 5'- aAGCAgUCUGCAUAUGC--AGGCAUCCa -3' miRNA: 3'- cUUGU-AGACGUGUGCGuuUCCGUAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 3517 | 0.71 | 0.951143 |
Target: 5'- gGAGCGUU---GC-CGUAGAGGCGUCCg -3' miRNA: 3'- -CUUGUAGacgUGuGCGUUUCCGUAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 154598 | 0.71 | 0.951143 |
Target: 5'- gGAGCGUU---GC-CGUAGAGGCGUCCg -3' miRNA: 3'- -CUUGUAGacgUGuGCGUUUCCGUAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 45031 | 0.7 | 0.959281 |
Target: 5'- -uACG-CUGCACAUGCuGAGGCAg-- -3' miRNA: 3'- cuUGUaGACGUGUGCGuUUCCGUagg -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 11192 | 0.69 | 0.980353 |
Target: 5'- -cACAUCguagaaucuUGCGCugGCucauGAAGGCcUCCa -3' miRNA: 3'- cuUGUAG---------ACGUGugCG----UUUCCGuAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 138039 | 0.68 | 0.987947 |
Target: 5'- uAACAUCUGCuaACGCGgCAAccGCAguUCCu -3' miRNA: 3'- cUUGUAGACG--UGUGC-GUUucCGU--AGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 1687 | 0.67 | 0.994845 |
Target: 5'- aGAGCGUCgacgGCGaGCGCGAcGGCcgCg -3' miRNA: 3'- -CUUGUAGa---CGUgUGCGUUuCCGuaGg -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 152768 | 0.67 | 0.994845 |
Target: 5'- aGAGCGUCgacgGCGaGCGCGAcGGCcgCg -3' miRNA: 3'- -CUUGUAGa---CGUgUGCGUUuCCGuaGg -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 10536 | 0.67 | 0.994845 |
Target: 5'- --uCGUC-GCACAUGaCGAAGGCAaaagaaUCCc -3' miRNA: 3'- cuuGUAGaCGUGUGC-GUUUCCGU------AGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 4317 | 0.67 | 0.996249 |
Target: 5'- uGACAUCgggguccGCAgACGCGguucccccgGGGGCGUCg -3' miRNA: 3'- cUUGUAGa------CGUgUGCGU---------UUCCGUAGg -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 155399 | 0.67 | 0.996249 |
Target: 5'- uGACAUCgggguccGCAgACGCGguucccccgGGGGCGUCg -3' miRNA: 3'- cUUGUAGa------CGUgUGCGU---------UUCCGUAGg -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 1571 | 0.66 | 0.997755 |
Target: 5'- ---gGUCUGCACcuCGCGAAGuGU-UCCg -3' miRNA: 3'- cuugUAGACGUGu-GCGUUUC-CGuAGG- -5' |
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4164 | 3' | -49.4 | NC_001664.1 | + | 152652 | 0.66 | 0.997755 |
Target: 5'- ---gGUCUGCACcuCGCGAAGuGU-UCCg -3' miRNA: 3'- cuugUAGACGUGu-GCGUUUC-CGuAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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