Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4173 | 5' | -58.2 | NC_001664.1 | + | 6434 | 0.66 | 0.816803 |
Target: 5'- -aCCUG-CGGCAcGGUcagagucauGCgCGCGGCCg -3' miRNA: 3'- aaGGACuGUCGU-CCAu--------CGgGCGCUGG- -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 157515 | 0.66 | 0.816803 |
Target: 5'- -aCCUG-CGGCAcGGUcagagucauGCgCGCGGCCg -3' miRNA: 3'- aaGGACuGUCGU-CCAu--------CGgGCGCUGG- -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 4815 | 0.66 | 0.808139 |
Target: 5'- --aCUGGCuGCuccUGGCCCGCGGCa -3' miRNA: 3'- aagGACUGuCGuccAUCGGGCGCUGg -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 155896 | 0.66 | 0.808139 |
Target: 5'- --aCUGGCuGCuccUGGCCCGCGGCa -3' miRNA: 3'- aagGACUGuCGuccAUCGGGCGCUGg -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 6505 | 0.69 | 0.632751 |
Target: 5'- gUCC---CAGgGGGUGGCCCGCGuacagguGCCg -3' miRNA: 3'- aAGGacuGUCgUCCAUCGGGCGC-------UGG- -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 157586 | 0.69 | 0.632751 |
Target: 5'- gUCC---CAGgGGGUGGCCCGCGuacagguGCCg -3' miRNA: 3'- aAGGacuGUCgUCCAUCGGGCGC-------UGG- -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 3812 | 1.09 | 0.001754 |
Target: 5'- cUUCCUGACAGCAGGUAGCCCGCGACCu -3' miRNA: 3'- -AAGGACUGUCGUCCAUCGGGCGCUGG- -5' |
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4173 | 5' | -58.2 | NC_001664.1 | + | 154893 | 1.09 | 0.001754 |
Target: 5'- cUUCCUGACAGCAGGUAGCCCGCGACCu -3' miRNA: 3'- -AAGGACUGUCGUCCAUCGGGCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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