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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 4174 | 5' | -59.6 | NC_001664.1 | + | 4007 | 1.09 | 0.00135 |
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Target: 5'- uCACACCCCCUGUCCCUAGCUCGCGACg -3' miRNA: 3'- -GUGUGGGGGACAGGGAUCGAGCGCUG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 155089 | 1.09 | 0.00135 |
|
Target: 5'- uCACACCCCCUGUCCCUAGCUCGCGACg -3' miRNA: 3'- -GUGUGGGGGACAGGGAUCGAGCGCUG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 6333 | 0.75 | 0.286776 |
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Target: 5'- -cCACCCCCUGggaCCgUGGCUCGUGuGCg -3' miRNA: 3'- guGUGGGGGACa--GGgAUCGAGCGC-UG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 157415 | 0.75 | 0.286776 |
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Target: 5'- -cCACCCCCUGggaCCgUGGCUCGUGuGCg -3' miRNA: 3'- guGUGGGGGACa--GGgAUCGAGCGC-UG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 2295 | 0.73 | 0.343033 |
|
Target: 5'- gACACCCCgCUGUCCgCUGuCUCGCGcCc -3' miRNA: 3'- gUGUGGGG-GACAGG-GAUcGAGCGCuG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 153377 | 0.73 | 0.343033 |
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Target: 5'- gACACCCCgCUGUCCgCUGuCUCGCGcCc -3' miRNA: 3'- gUGUGGGG-GACAGG-GAUcGAGCGCuG- -5' |
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| 4174 | 5' | -59.6 | NC_001664.1 | + | 101315 | 0.72 | 0.40682 |
|
Target: 5'- aCGCACCCUacaauCUGUCC--AGCUUGCGAUa -3' miRNA: 3'- -GUGUGGGG-----GACAGGgaUCGAGCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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