Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4175 | 5' | -59 | NC_001664.1 | + | 4316 | 1.05 | 0.003077 |
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Target: 5'- uUGACAUCGGGGUCCGCAGACGCGGUUc -3' miRNA: 3'- -ACUGUAGCCCCAGGCGUCUGCGCCAA- -5' |
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| 4175 | 5' | -59 | NC_001664.1 | + | 155398 | 1.05 | 0.003077 |
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Target: 5'- uUGACAUCGGGGUCCGCAGACGCGGUUc -3' miRNA: 3'- -ACUGUAGCCCCAGGCGUCUGCGCCAA- -5' |
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| 4175 | 5' | -59 | NC_001664.1 | + | 4358 | 0.71 | 0.486875 |
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Target: 5'- cGACGUCGGu-UCCGCAGAaaacacCGCGGUc -3' miRNA: 3'- aCUGUAGCCccAGGCGUCU------GCGCCAa -5' |
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| 4175 | 5' | -59 | NC_001664.1 | + | 155440 | 0.71 | 0.486875 |
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Target: 5'- cGACGUCGGu-UCCGCAGAaaacacCGCGGUc -3' miRNA: 3'- aCUGUAGCCccAGGCGUCU------GCGCCAa -5' |
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| 4175 | 5' | -59 | NC_001664.1 | + | 6212 | 0.69 | 0.593802 |
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Target: 5'- aGACcgUGGGaGaUCCGUGGACgGCGGUUc -3' miRNA: 3'- aCUGuaGCCC-C-AGGCGUCUG-CGCCAA- -5' |
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| 4175 | 5' | -59 | NC_001664.1 | + | 157294 | 0.69 | 0.593802 |
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Target: 5'- aGACcgUGGGaGaUCCGUGGACgGCGGUUc -3' miRNA: 3'- aCUGuaGCCC-C-AGGCGUCUG-CGCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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