Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4179 | 5' | -50.6 | NC_001664.1 | + | 9663 | 1.08 | 0.011132 |
Target: 5'- gAACGAACGCCGUCCAUUUAACCUCCGg -3' miRNA: 3'- -UUGCUUGCGGCAGGUAAAUUGGAGGC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 10663 | 0.8 | 0.493694 |
Target: 5'- uGCGGACGCCGUCCAccucgggguCCUCCa -3' miRNA: 3'- uUGCUUGCGGCAGGUaaauu----GGAGGc -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 6401 | 0.7 | 0.949793 |
Target: 5'- --gGAAcCGCCGUCCAcg-GAUCUCCc -3' miRNA: 3'- uugCUU-GCGGCAGGUaaaUUGGAGGc -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 157482 | 0.7 | 0.949793 |
Target: 5'- --gGAAcCGCCGUCCAcg-GAUCUCCc -3' miRNA: 3'- uugCUU-GCGGCAGGUaaaUUGGAGGc -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 127775 | 0.68 | 0.979286 |
Target: 5'- aAGCGAA-GCC-UCCAgcagAGCCUCCa -3' miRNA: 3'- -UUGCUUgCGGcAGGUaaa-UUGGAGGc -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 8496 | 0.67 | 0.991288 |
Target: 5'- gAACGAccGCgGCCGUUCucucu-CCUCCGg -3' miRNA: 3'- -UUGCU--UG-CGGCAGGuaaauuGGAGGC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 9653 | 0.67 | 0.990032 |
Target: 5'- uACGGAUGCUGUCCcgguaugGAUCUCaCGg -3' miRNA: 3'- uUGCUUGCGGCAGGuaaa---UUGGAG-GC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 2204 | 0.66 | 0.993424 |
Target: 5'- cGACGAcgaGCCGUCCGUccgcCCUCgGu -3' miRNA: 3'- -UUGCUug-CGGCAGGUAaauuGGAGgC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 153286 | 0.66 | 0.993424 |
Target: 5'- cGACGAcgaGCCGUCCGUccgcCCUCgGu -3' miRNA: 3'- -UUGCUug-CGGCAGGUAaauuGGAGgC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 63252 | 0.66 | 0.99644 |
Target: 5'- uGACGAGCGCUuguacguugGcUCCAUUUAugugucuCCUUCGa -3' miRNA: 3'- -UUGCUUGCGG---------C-AGGUAAAUu------GGAGGC- -5' |
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4179 | 5' | -50.6 | NC_001664.1 | + | 12193 | 0.66 | 0.993424 |
Target: 5'- aAugGAGCGuuGcaucgauaCCGUUucUGGCCUCCGc -3' miRNA: 3'- -UugCUUGCggCa-------GGUAA--AUUGGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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