Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
423 | 3' | -53.9 | AC_000011.1 | + | 8262 | 0.7 | 0.374626 |
Target: 5'- cGGCGGCGCGCGguugacuuGCAGgaguuuuuccagggcGCGCGGGAGg- -3' miRNA: 3'- -UCGUCGUGUGU--------UGUC---------------CGCGUUCUCag -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 35041 | 0.71 | 0.335132 |
Target: 5'- uGGCuGUuauuuuccaccaACACAGCGGGgGCGAGAGg- -3' miRNA: 3'- -UCGuCG------------UGUGUUGUCCgCGUUCUCag -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 20791 | 0.72 | 0.301944 |
Target: 5'- uGGCccuGGCGCAUggUGGGCGCGAG-GUa -3' miRNA: 3'- -UCG---UCGUGUGuuGUCCGCGUUCuCAg -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 17642 | 0.73 | 0.257008 |
Target: 5'- cAGCAcCACAgGGCAGGCGCuGAGGGa- -3' miRNA: 3'- -UCGUcGUGUgUUGUCCGCG-UUCUCag -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 24996 | 0.74 | 0.200038 |
Target: 5'- gGGCAGCGCGgAGCAGGUggcggGCAGGAuUCc -3' miRNA: 3'- -UCGUCGUGUgUUGUCCG-----CGUUCUcAG- -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 12764 | 0.83 | 0.053659 |
Target: 5'- cAGCAGCA-GCAGCAGGCGCGAGuucggcGUCa -3' miRNA: 3'- -UCGUCGUgUGUUGUCCGCGUUCu-----CAG- -5' |
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423 | 3' | -53.9 | AC_000011.1 | + | 36081 | 1.1 | 0.00047 |
Target: 5'- cAGCAGCACACAACAGGCGCAAGAGUCa -3' miRNA: 3'- -UCGUCGUGUGUUGUCCGCGUUCUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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