Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4240 | 5' | -58.8 | NC_001702.1 | + | 4134 | 0.67 | 0.091447 |
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Target: 5'- uGGUUCCaUGCgUUUcaGCCAAGAUCUCg -3' miRNA: 3'- uCCGAGG-ACGgGAG--UGGUUCUAGGGa -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 3397 | 0.67 | 0.088222 |
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Target: 5'- uGGGCUuucuuggCCgagGCCCgguauCCGAGGUCCCc -3' miRNA: 3'- -UCCGA-------GGa--CGGGagu--GGUUCUAGGGa -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 3554 | 0.69 | 0.061617 |
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Target: 5'- gAGGCggcagaagCCUGCCgUCcCUAGGAacUCCCUu -3' miRNA: 3'- -UCCGa-------GGACGGgAGuGGUUCU--AGGGA- -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 3799 | 0.7 | 0.04566 |
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Target: 5'- uAGGC-CacagGCCgaCGCCAAGGUCCCa -3' miRNA: 3'- -UCCGaGga--CGGgaGUGGUUCUAGGGa -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 3531 | 0.73 | 0.025779 |
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Target: 5'- cAGGCUUCUGCC---GCCucuGGAUCCCUg -3' miRNA: 3'- -UCCGAGGACGGgagUGGu--UCUAGGGA- -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 2281 | 0.74 | 0.023292 |
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Target: 5'- gGGGCUCCUGaCCCUgACC----UCCCUa -3' miRNA: 3'- -UCCGAGGAC-GGGAgUGGuucuAGGGA- -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 1687 | 0.75 | 0.018375 |
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Target: 5'- gGGGCUCCUGCCCgCAUUuugGAGA-CCCg -3' miRNA: 3'- -UCCGAGGACGGGaGUGG---UUCUaGGGa -5' |
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| 4240 | 5' | -58.8 | NC_001702.1 | + | 5881 | 1.08 | 2.4e-05 |
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Target: 5'- gAGGCUCCUGCCCUCACCAAGAUCCCUa -3' miRNA: 3'- -UCCGAGGACGGGAGUGGUUCUAGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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