Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4319 | 3' | -53.9 | NC_001720.1 | + | 13891 | 0.66 | 0.769327 |
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Target: 5'- -uUAUCUGcacgcuaaaGGAcCCCGAAGCCCGuACa -3' miRNA: 3'- guAUGGACa--------CCU-GGGCUUUGGGC-UGc -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 24971 | 0.66 | 0.748452 |
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Target: 5'- --gGCCaucGUGGACCCGAcagggcgccuCCaCGGCGa -3' miRNA: 3'- guaUGGa--CACCUGGGCUuu--------GG-GCUGC- -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 23631 | 0.67 | 0.70533 |
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Target: 5'- --gACUUcGUGGACCuCGAAgaGCCCGGg- -3' miRNA: 3'- guaUGGA-CACCUGG-GCUU--UGGGCUgc -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 25706 | 0.67 | 0.691024 |
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Target: 5'- -cUACCUGUGGGaagcCCCGGAggaagaggccaacaACCCGuCc -3' miRNA: 3'- guAUGGACACCU----GGGCUU--------------UGGGCuGc -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 24176 | 0.67 | 0.661022 |
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Target: 5'- --cACCaGUGGACgaGGAGCCCcagGACGa -3' miRNA: 3'- guaUGGaCACCUGggCUUUGGG---CUGC- -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 37428 | 0.68 | 0.638655 |
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Target: 5'- gGUcCCUGUGGACCCu-AGCCa-GCGg -3' miRNA: 3'- gUAuGGACACCUGGGcuUUGGgcUGC- -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 6227 | 0.71 | 0.45485 |
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Target: 5'- uCGUGCaacaUGuUGGACCCGAucCCCGAa- -3' miRNA: 3'- -GUAUGg---AC-ACCUGGGCUuuGGGCUgc -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 14906 | 0.71 | 0.444843 |
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Target: 5'- --gGCC-GUGGGCCUcc-GCCCGACGa -3' miRNA: 3'- guaUGGaCACCUGGGcuuUGGGCUGC- -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 38233 | 0.71 | 0.425206 |
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Target: 5'- gGUAgggGUGGugCCGAGACCCGAg- -3' miRNA: 3'- gUAUggaCACCugGGCUUUGGGCUgc -5' |
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| 4319 | 3' | -53.9 | NC_001720.1 | + | 1313 | 1.08 | 0.001376 |
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Target: 5'- cCAUACCUGUGGACCCGAAACCCGACGc -3' miRNA: 3'- -GUAUGGACACCUGGGCUUUGGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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