Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4324 | 5' | -58.5 | NC_001720.1 | + | 12317 | 0.67 | 0.398617 |
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Target: 5'- aUGCCUaucCCGGcgcGGGCgCGACACCCa -3' miRNA: 3'- aAUGGGa--GGCUu--CUCGgGCUGUGGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 21314 | 0.68 | 0.329528 |
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Target: 5'- ---aCCUCCGGAG-GCCCGGgAgCCg -3' miRNA: 3'- aaugGGAGGCUUCuCGGGCUgUgGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 2550 | 0.68 | 0.321539 |
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Target: 5'- uUUugCCUCCuGAGGGCUugCGugGCCCc -3' miRNA: 3'- -AAugGGAGGcUUCUCGG--GCugUGGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 11397 | 0.69 | 0.305999 |
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Target: 5'- -aAgCCUUCGcGGAGCuaGACACCCa -3' miRNA: 3'- aaUgGGAGGCuUCUCGggCUGUGGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 28490 | 0.69 | 0.298447 |
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Target: 5'- -aGCCCgucUCCGggGgugAGgCUGACGCCCa -3' miRNA: 3'- aaUGGG---AGGCuuC---UCgGGCUGUGGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 7548 | 0.69 | 0.27666 |
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Target: 5'- -gACCCUCgCGAcGGGCCCuuauAUACCCg -3' miRNA: 3'- aaUGGGAG-GCUuCUCGGGc---UGUGGGa -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 39746 | 0.72 | 0.171782 |
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Target: 5'- ---aCCUCCGGAGugcaAGUCCGACGCUCUu -3' miRNA: 3'- aaugGGAGGCUUC----UCGGGCUGUGGGA- -5' |
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| 4324 | 5' | -58.5 | NC_001720.1 | + | 4221 | 1.04 | 0.00068 |
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Target: 5'- gUUACCCUCCGAAGAGCCCGACACCCUc -3' miRNA: 3'- -AAUGGGAGGCUUCUCGGGCUGUGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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