Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4326 | 3' | -54.7 | NC_001720.1 | + | 10889 | 0.65 | 0.698234 |
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Target: 5'- cGACUUCUACCGcaugcucugaccgcGCGCGaCGCgGGaCUc -3' miRNA: 3'- cCUGAAGAUGGU--------------CGCGCaGUGgUC-GA- -5' |
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| 4326 | 3' | -54.7 | NC_001720.1 | + | 38569 | 0.66 | 0.668143 |
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Target: 5'- uGGACUUUgACCca-GCGUCACUAgGCg -3' miRNA: 3'- -CCUGAAGaUGGucgCGCAGUGGU-CGa -5' |
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| 4326 | 3' | -54.7 | NC_001720.1 | + | 9487 | 0.66 | 0.6659 |
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Target: 5'- aGGGCUUugagacggucgucCUGCCgugcuaagcguucGGCGCGggagACCAGCUg -3' miRNA: 3'- -CCUGAA-------------GAUGG-------------UCGCGCag--UGGUCGA- -5' |
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| 4326 | 3' | -54.7 | NC_001720.1 | + | 31093 | 0.66 | 0.644548 |
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Target: 5'- gGGACUUUgcaacugUACCaAGCGCc-CACUAGCa -3' miRNA: 3'- -CCUGAAG-------AUGG-UCGCGcaGUGGUCGa -5' |
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| 4326 | 3' | -54.7 | NC_001720.1 | + | 11140 | 0.7 | 0.45007 |
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Target: 5'- aGGGCgcagGCCGGUGCGgucgguuaccCGCCGGCUg -3' miRNA: 3'- -CCUGaagaUGGUCGCGCa---------GUGGUCGA- -5' |
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| 4326 | 3' | -54.7 | NC_001720.1 | + | 6514 | 1.1 | 0.000668 |
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Target: 5'- gGGACUUCUACCAGCGCGUCACCAGCUg -3' miRNA: 3'- -CCUGAAGAUGGUCGCGCAGUGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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